enzymology Training Program
Rapture
Biotech is a Biotechnology Company, providing hands on Industrial project Training
in the field of Cancer Drug Research, Clinical Microbiology, Bioinformatics,
Plant Tissue Culture, Biopharma Wet Lab Analytics, Biochemistry, Cell Biology,
Environment Biotechnology etc.
Course
Module of Enzymology Biotech Training Program Duration – 5-6 Months
Cell disruption methods
Enzyme assay
Enzyme kinetics
Week-1
Quantitative
estimation of protein by lowery method
Quantitative estimation of protein by bradfords method
Substrate concentration activators & Inhibitors Week-2
--------------------------------------------------------------------------------------------------------------------
Separation &
identification of a.a by TLC
Isolation
of enzyme using native-PAGE
Demonstrating
the presence of catalase
Effects of
pH/Temperature Week-3
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Extraction of enzyme
& proteins
Preparation of standard
curve
Enzyme concentration
Computer Simulation of Enzyme
Activity Week-4
Kinetic
analysis
Determination
of Km & Vmax
Addition
of enzyme inhibitors
Protein
concentration/enzyme activity Week-5
------------------------------------------------------------------------------------------------------------------------ Studying
the action & activity of amylase on starch digestion
Determination
of the effect of pH on the activity of human salivary a-amylase
Determining
the effect of temperature on the activity of human salivary of a-amylase
Construction
of the maltose calibration curve Week-6
Training
Help Line No. – 9027326396
Address
- Rapture
Biotech, 66, Dev Nagar, Khandari Bypass, NH-2 Agra-
282005(U.P)
E-mail - info@rapturebio.com, rapturebio@gmail.com
Phone -
+91-9219567153, Office - 0562 – 4063439; Website -
www.rapturebio.com
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